- Title
- Insertions within the Saxitoxin Biosynthetic Gene Cluster Result in Differential Toxin Profiles
- Creator
- Cullen, Alescia; D'Agostino, Paul M.; Mazmouz, Rabia; Pickford, Russell; Wood, Susanna; Neilan, Brett A.
- Relation
- ACS Chemical Biology Vol. 13, Issue 11, p. 3107-3114
- Publisher Link
- http://dx.doi.org/10.1021/acschembio.8b00608
- Publisher
- American Chemical Society (ACS)
- Resource Type
- journal article
- Date
- 2018
- Description
- The neurotoxin saxitoxin and related paralytic shellfish toxins are produced by multiple species of cyanobacteria and dinoflagellates. This study investigates the two saxitoxin-producing strains of Scytonema crispum, CAWBG524 and CAWBG72, isolated in New Zealand. Each strain was previously reported to have a distinct paralytic shellfish toxin profile, a rare observation between strains within the same species. Sequencing of the saxitoxin biosynthetic clusters (sxt) from S. crispum CAWBG524 and S. crispum CAWBG72 revealed the largest sxt gene clusters described to date. The distinct toxin profiles of each strain were correlated to genetic differences in sxt tailoring enzymes, specifically the open-reading frame disruption of the N-21 sulfotransferase sxtN, adenylylsulfate kinase sxtO, and the C-11 dioxygenase sxtDIOX within S. crispum CAWBG524 via genetic insertions. Heterologous overexpression of SxtN allowed for the proposal of saxitoxin and 3′-phosphoadenosine 5′-phosphosulfate as substrate and cofactor, respectively, using florescence binding assays. Further, catalytic activity of SxtN was confirmed by the in vitro conversion of saxitoxin to the N-21 sulfonated analog gonyautoxin 5, making this the first known report to biochemically confirm the function of a sxt tailoring enzyme. Further, SxtN could not convert neosaxitoxin to its N-21 sulfonated analog gonyautoxin 6, indicating paralytic shellfish toxin biosynthesis most likely occurs along a predefined route. In this study, we identified key steps toward the biosynthetic conversation of saxitoxin to other paralytic shellfish toxins.
- Subject
- genes; toxin profiles; saxitoxin biosynthetic; SDG 2; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1453374
- Identifier
- uon:44660
- Identifier
- ISSN:1554-8929
- Language
- eng
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